|
Calendar of Physics Talks Vienna
| Nanoscopic Anchors for Antibodies: Form Nano-Bio-Sensors to Single Antibody Presentation |
| Speaker: | Univ.Prof. Dr. Thomas A. Klar (Johannes-Kepler-Universität Linz, Institut für Angewandte Physik, Linz) |
| Abstract: | The ability to place individual proteins onto nano-confined structures plays a constantly growing role in bioscience, from basic studies in biology to the development of nanosensors. One of the possibilities to generate sub-micrometre sized structures is direct laser writing (DLW) lithography. The resolution of DLW can be enhanced by stimulated emission depletion (STED) for assembly of polymeric structures down to several tens of nanometres [1]. Using a pulsed 780nm laser for two-photon DLW and a 532nm laser for STED, we are able to obtain structure sizes of down to 55nm and manufacture two clearly separated lines with 120nm distance [2]. The structures show good biocompatibility and allow an easy bio-functionalization with proteins down to the single protein level. We use direct stochastic optical reconstruction microscopy (dSTORM), which enables determination of protein density at a na |
| Date: | Tue, 26.03.2013 |
| Time: | 16:00 |
| Location: | Technische Universität Wien, Institut für Angewandte Physik, Seminarraum 134A, Turm B (gelbe Leitfarbe), 5. OG, 1040 Wien, Wiedner Hauptstraße 8-10 |
| Contact: | Univ.Prof. Dr. Gerhard Schütz |
|